Skip to main content
. 2013 Dec 26;289(8):4896–4905. doi: 10.1074/jbc.M113.514869

FIGURE 4.

FIGURE 4.

CnAβ regulates expression of Nox isoforms. A, Nox1, Nox2, and Nox4 protein expression was examined in WT and CnAβ−/− kidney fibroblasts by Western blot following 48 h of NG or HG treatment. Data shown are representative of four independent experiments. Nox2 (B) and Nox4 (C) protein expression was quantitated by densitometry, normalized to WT NG, and graphed. Data are ± S.E. (error bars) normalized to WT NG. *, p < 0.05; #, p < 0.05; ##, p < 0.01 compared with WT NG. Nox2 (D) and Nox4 (E) mRNA expression was examined in WT and CnAβ−/− fibroblasts exposed to NG or HG for 48 h by qRT-PCR. Data shown are the mean ± S.E. of 3–6 replicates/group normalized to WT NG. *, p < 0.05; **, p < 0.01 compared with NG; #, p < 0.05 compared with NG WT. F, Nox2 and Nox4 protein expression was examined in whole kidney lysates from WT and CnAβ−/− mice by Western blot analysis. Data shown are representative of four independent experiments.