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. 2013 Dec 26;289(8):4896–4905. doi: 10.1074/jbc.M113.514869

FIGURE 7.

FIGURE 7.

CnAβ regulates NFAT transcriptional activity. A, NFAT activity in WT, CnAα−/−, CnAβ−/−, and CnAβ−/−B kidney fibroblasts exposed to low or high serum was examined using an NFAT-responsive luciferase promoter construct. Data shown are the mean ± S.E. (error bars) of 5 replicates/group. *, p < 0.05; **, p < 0.01 compared with WT control. B, ROS generation was examined by an Amplex Red assay in WT kidney fibroblasts treated with an increasing concentration of VIVIT peptide and then exposed to NG or HG for 48 h. Data shown are the mean ± S.E. of 8 replicates/group. **, p < 0.01 compared with NG. Nox2 (C) and Nox4 (D) mRNA expression in WT kidney fibroblasts treated with 10 nm VIVIT were examined by qRT-PCR. Data shown are the mean ± S.E. of triplicate samples per group. *, p < 0.05 compared with control. E, basal Nox2 and Nox4 mRNA expression in vector- or NFAT-DN-transfected WT kidney fibroblasts were examined by qRT-PCR. Data shown are the mean ± S.E. of triplicate samples per group. **, p < 0.01 compared with vector. F, basal Nox2 and Nox4 mRNA expression in vector- or NFAT-transfected WT kidney fibroblasts were examined by qRT-PCR. Data shown are the mean ± S.E. of triplicate samples per group. *, p < 0.05 compared with vector. ALU, arbitrary light units.