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. 2013 Dec 29;289(8):4941–4951. doi: 10.1074/jbc.M113.530808

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FIGURE 2. — A, SDS-PAGE of SCl-Fn recombinant collagen constructs. Top panel from left to right: marker, SCl-Fn_G784-R792, SCl-Fn_G781-R792, SCl-Fn_G778-R792, and SCl-Fn_G775-R792. Bottom panel from left to right: SCl-Fn_G772-V783, marker, SCl-Fn_G769-P786, SCl-Fn_G769-R789, marker, and SCl-Fn_G769-R792. Lower bands in SCl-Fn_G769-R789 and SCl-Fn_G769-R792 represent truncated products with a single CL unit. Triple-helical proteins migrate slower than normal in SDS-PAGE, thus the M_r prediction from the protein standard is higher than the actual M_w. B, examples of MALDI-TOF mass spectra showing M_r of SCl-Fn. SCl-Fn_G775-R792, top panel; and SCl-Fn_G769-R792, bottom panel.