FIGURE 10.

FOS knockdown affects FOSL1 accumulation. A, Swan 71 trophoblast cells expressing control shRNA (shCTRL) or shRNAs targeting FOS, FOSB, or FOSL1 were plated on Matrigel for 1 h. Proteins were subsequently extracted and analyzed by Western blotting for FOS, FOSB, FOSL1, or phospho-Ser²⁶⁵-FOSL1 (P-FOSL1). α-Tubulin was used as a loading control. B, FOSL1 transcript levels in control shRNA (shCTRL) or shRNA targeting FOS. C, relative mRNA expression comparing FOS and FOSL1 in trophoblast cells ectopically expressing FOS. D, Western blot showing FOS, FOSL1, or P-FOSL1 in trophoblast cells ectopically expressing FOS. α-Tubulin served as a loading control. Values significantly different (p < 0.05) from control are denoted with an asterisk (*). Error bars represent S.E.