FIGURE 2.

Effect of scratch wounding on expression of FOS family members in trophoblast cells. A, relative mRNA expression of FOS, FOSB, FOSL1, and FOSL2 during standard plating conditions (unwounded) and 1, 3, and 6 h following scratch wounding in Swan 71 trophoblast cells (left) and HTR8 trophoblast cells (right). 18 S rRNA was used as a loading control for transcript. B, protein expression of FOS, FOSB, FOSL1, phospho-Ser²⁶⁵-FOSL1 (P-FOSL1), and FOSL2 during standard plating conditions (unwounded) and 1, 3, and 6 h following scratch wounding. α-Tubulin was used as a loading control for protein. C, immunocytochemistry for FOS, FOSB, and FOSL1 in unwounded versus wounded monolayers. The wound is shown using a double-sided arrow. Values significantly different (p < 0.05) from controls are denoted with an asterisk (*). Error bars represent S.E.