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. 2013 Dec 30;289(8):5025–5039. doi: 10.1074/jbc.M113.523746

FIGURE 3.

FIGURE 3.

ERK-MAPK regulates wounding-induced FOS, FOSB, and FOSL1. A, the left four lanes of Western blot show levels of phospho-ERK MAPK, phospho-p38 MAPK, and phospho-JNK MAPK in unwounded cells and 10, 30, and 60 min following wounding, and the right four lanes show efficacy of MAPK inhibitors U0126 (U0) (10 μm), SB203580 (SB) (20 μm), and SP600125 (SP) (20 μm) on phosphorylated MAPK levels 30 min postwound. α-Tubulin was used as a loading control. Vehicle (V) only (DMSO, 1:1000) was used as a control. FOS (B) and FOSB (C) mRNA following treatment with U0126 (10 μm), SB203580 (20 μm), and SP600125 (20 μm) is shown. Vehicle (V) only (DMSO, 1:1000) was used as a control. D, FOS, FOSB, FOSL1, and phospho-Ser265-FOSL1 (P-FOSL1) expression following treatment with vehicle (V; DMSO, 1:1000) or U0126 (10 μm) in unwounded cells or 1 or 3 h postwounding. Values significantly different (p < 0.05) from controls are denoted with an asterisk (*). Error bars represent S.E.