FIGURE 4.

FOS, FOSB, and FOSL1 are induced when placed on Matrigel. A, relative mRNA levels of FOS, FOSB, FOSL1, and FOSL2 expressed by Swan 71 cells in quiescent (standard cell culture) conditions versus plating on Matrigel for 1, 3, or 6 h. B, Western blot showing protein levels of FOS, FOSB, FOSL1, phospho-Ser²⁶⁵-FOSL1 (P-FOSL1), and FOSL2 in quiescent (standard cell culture; Q) conditions or following 1, 3, 6, or 24 h of plating on Matrigel. α-Tubulin was used as a loading control for protein. Values significantly different (p < 0.05) from controls are denoted with an asterisk (*). Error bars represent S.E.