FOS family members differentially affect expression of MMPs following plating on Matrigel.
A, relative mRNA expression of MMP1, MMP3, and MMP10 in Swan 71 trophoblast cells expressing control shRNA (shCTRL) or shRNAs for FOS, FOSB, or FOSL1 after plating on Matrigel for 6 h. B, relative occupancy of FOSL1 on the promoter regions of MMP1, MMP3, and MMP10. A diagram depicting the primer sites in relation to the characterized AP-1 sites is indicated. Values significantly increased (p < 0.05) from controls are denoted with an asterisk (*), whereas values significantly decreased (p < 0.05) from controls are denoted with a double asterisk (**). Error bars represent S.E.