FIGURE 3.

Dok-2 negatively regulates integrin α_IIbβ₃-dependent platelet adhesion under shear conditions. Anticoagulated whole blood from WT or Dok-2^−/− mice was perfused through fibrinogen (20 or 100 μg/ml)-coated microslides at 600 s⁻¹. A, the number of WT (●) or Dok-2^−/− (○) platelets adherent (≥2 s) to fibrinogen (20 μg/ml) during whole blood perfusion (mean ± S.E.; n = 4; *, p < 0.05). Quantification of platelet adhesion was performed as described under “Experimental Procedures.” B, representative cropped images taken from one paired flow on a fibrinogen matrix (100 μg/ml) following modified Tyrode buffer washout. Scale bar = 10 μm. C, the total surface area coverage of adherent platelets. These data are taken from three random fields per experiment and are representative of five independent experiments (mean ± S.E.).