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Gt activation by photoactivated bvRh (A), mRh (B), cG (C), and mG (D) in POPC/POPG nanodiscs. 20 nm pigment was mixed with 400 nm Gt and 100 μm GTPγS at 37 °C. The mixture was irradiated with a yellow flash at time 0, and the fluorescence intensity was monitored (upper traces). The fluorescence increase that originated from opsin formation was monitored in the absence of Gt at 37 °C (lower traces).
