Initial velocities of Gt activation by photoactivated pigments in POPG/POPG nanodiscs. Initial velocities of bvRh, cG, mRh, and mG (closed squares, closed circles, open squares, and open circles, respectively) at 0 (A), 10 (B), 20 (C), and 37 °C (D) were plotted against Gt concentration and fitted by the Michaelis-Menten equation or Equation 29 (solid lines for bvRh and mG and dashed lines for mRh and cG) to estimate Vmax/R*. Error bars represent S.D. estimated from three independent measurements.