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. 2013 Dec 27;289(8):5061–5073. doi: 10.1074/jbc.M113.508507

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© 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Initial velocities of G_t activation by photoactivated bvRh (A) and mG (B) in POPC/POPG, POPC, and DOPC/POPG nanodiscs. Initial velocities of bvRh and mG in POPC/POPG, POPC, and DOPC/POPG nanodiscs (closed squares, closed circles, and closed triangles, respectively) at 37 °C were plotted against G_t concentration and fitted by the Michaelis-Menten equation or Equation 29 (solid lines for POPC/POPG nanodiscs, dashed lines for POPC nanodiscs, and dotted lines for DOPC/POPG nanodiscs) to estimate V_max/R*. Error bars represent S.D. estimated from three independent measurements.