FIGURE 6.
Photobleaching processes and estimation of Meta-II ratios of bvRh. The b-spectra of bvRh in POPC/POPG nanodiscs at 20 °C (A), in ROSs at 20 °C (B), and in DM suspension at 20 °C (C) calculated by SVD analysis are presented. The b-spectra of bvRh in nanodiscs at 0 and 10 °C were consistent with those at 20 °C. The b1 spectrum of bvRh in nanodiscs at 37 °C was consistent with that at 20 °C. Two-step formation of Meta-II (b1 and b2) is consistent with previous reports that suggested the presence of two forms of Meta-I (Meta-Ia and Meta-Ib) (32, 45). b0 spectra, which were difference spectra in the equilibrium state, of bvRh in nanodiscs and ROSs and DM suspension are shown in D (solid lines for bvRh in nanodiscs at 0–37 °C and dashed lines for bvRh in ROSs or DM suspension at 20 °C). These spectra were fitted by Meta-I and Meta-II model spectra as described previously (36, 37) to estimate FMeta-II (Table 3). Diff. Abs., difference absorbance; Rel. Diff. Abs., relative difference absorbance.