TABLE 1.

Kinetic parameters of G_t activation by rhodopsin and cone visual pigments

Values are shown as average ± S.D. calculated from three independent measurements. ND, not determined.

Sample	Temperature	Vmax/R*	Vmax/Meta-II	Km or K
	°C	s−1	s−1	nm
ROSa	20	13.8 ± 0.9	24.2 ± 1.5	774 ± 60
bvRh/nanodisca	20	11.9 ± 1.3	23.3 ± 2.5	718 ± 152
bvRh/nanodisc	0	2.42 ± 0.27	17.3 ± 1.9	638 ± 227
	10	5.42 ± 0.98	17.5 ± 3.2	465 ± 117
	20	11.2 ± 1.7	22.0 ± 3.3	595 ± 45
	37	38.6 ± 3.3	52.2 ± 4.5	1158 ± 120
mRh/nanodisc	20	11.3 ± 1.6	ND	731 ± 142
	37	38.8 ± 1.2	ND	856 ± 124
cG/nanodisc	0	1.56 ± 0.21b	3.90 ± 0.53c	363 ± 108
	10	3.96 ± 0.24b	7.62 ± 0.46c	841 ± 89
	20	8.21 ± 0.79b	13.5 ± 1.3c	932 ± 45
	37	21.1 ± 1.9b	28.5 ± 2.6c	901 ± 130
mG/nanodisc	20	4.73 ± 0.69b,d	12.1 ± 1.8c	1068 ± 177
	37	15.4 ± 1.2b,d	37.6 ± 3.0c	794 ± 153

^a The concentration of bvRh was 2 nm. In other experiments, the concentrations of pigments were 20 nm. Lipid composition of nanodiscs was a mixture of POPC/POPG at a ratio of 3:2 (POPC/POPG).

^b Significantly different from V_max/R* of bvRh and mRh at the same temperature (p < 0.05).

^c Significantly different from V_max/Meta-II of bvRh at the same temperature (p < 0.05).

^d Significantly different from V_max/R* of cG at the same temperature (p < 0.05).