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. 2013 Dec 30;289(8):5083–5096. doi: 10.1074/jbc.M113.532929

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FIGURE 7. — Analysis of SPR association kinetic data at 25 °C. k_obs was obtained after the fitting of a two-exponential function to the association data from the sensorgrams versus m157 concentration. A, k_obs1 versus m157 concentration corresponding to the slow exponential function component for the Ly49H/m157 and Ly49I/m157 couples. These distributions correspond to a conformational selection mechanism. B, k_obs2 versus m157 concentration corresponding to the fast exponential function component for the two interactions studied. The linear dependence of the points indicates a simple 1:1 interaction of the Ly49*/m157 complex with a second m157 ligand.