FIGURE 3.

TRAX-translin association is not affected by PLCβ or oligonucleotides. A, FRET studies monitoring the decrease in fluorescence intensity (F.I.) in arbitrary units of 2 nm Alexa488-TRAX (FRET donor) and as Alexa546-translin (FRET acceptor) are added (●) in the absence or presence (▿) of 100 nm PLCβ. Although initially PLCβ1 was used, the data presented used PLCβ3. Both gave identical results. The data presented are a compilation of n = 9 experiments, and S.D. is shown. B, identical FRET study as described in A comparing the association between Alexa488-TRAX and Alexa546-translin in the absence (●) or presence (○) of 100 nm ssDNA where n = 3, and S.D. is shown.