FIGURE 2.

ANP prevents thrombin-induced disassembly of peripheral MTs. A, cells grown on coverslips were stimulated with thrombin (0.5 units/ml, 15 min) with or without ANP pretreatment (100 nm, 20 min) followed by immunofluorescent staining with an antibody against β-tubulin (top) or Texas Red-phalloidin to visualize F-actin (middle). Merged images of β-tubulin and F-actin staining are shown in the bottom panel. Bar, 5 μm. B, quantification of peripheral microtubules. *, p < 0.05, thrombin + ANP versus thrombin. Error bars, S.D.