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ANP prevents peripheral MT network disassembly caused by thrombin. A, cells were pretreated with ANP (100 nm, 20 min) followed by thrombin (0.5 units/ml) stimulation for 15 min. Endogenous EB1 was visualized by immunostaining with anti-EB1 antibody. Bar, 5 μm. B, quantification of peripheral EB1 in methanol-fixed HPAEC monolayers; *, p < 0.05, thrombin + ANP versus thrombin. Error bars, S.D.
