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. 2013 Dec 18;289(8):5168–5183. doi: 10.1074/jbc.M113.493924

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FIGURE 6. — Molecular inhibition of PAK1 abrogates protective effects of ANP on thrombin-induced peripheral MT disassembly, actin stress fiber formation, and endothelial permeability. Analysis of MT remodeling (A) and F-actin remodeling (B) in ECs expressing dominant negative PAK1 (myc-PAK1-DN) was performed by double immunofluorescence staining with either β-tubulin or Texas Red phalloidin and Myc antibody to detect PAK1-DN-expressing cells. Bar, 10 μm. Magnified images (insets) show details of MT and actin structure in non-transfected and PAK1-DN-expressing cells. Bar graphs depict the quantitative analysis of F-actin content. *, p < 0.05 versus transfected cells. C, transendothelial resistance reflecting EC monolayer barrier properties was monitored over 3 h in control and PAK1-DN-transfected ECs stimulated with thrombin or ANP + thrombin. Data are expressed as mean ± S.D. (error bars).