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Effects of pH and temperature on the activity of HypBA1. A, pH dependence of HypBA1 activity in various buffers at 37 °C for 10 min. Enzyme activities were expressed as the percentage of activity in sodium acetate buffer at pH 4.5. B, temperature dependence of HypBA1 activity for 10 min. Enzyme activities were expressed as the percentage of the activity at 35 °C. Buffers: sodium acetate (closed square), MES (open circle), sodium phosphate (closed circle).
