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. 2014 Jan 2;289(8):5240–5249. doi: 10.1074/jbc.M113.528711

FIGURE 8.

FIGURE 8.

Comparison of the reactivities of HypBA1 and HypBA2 with glycoproteins. A, TLC analysis of the reaction products. Potato lectin (lane 2), extensin (lane 3), and Ara3-Hyp (lane 4) were incubated with HypBA2-CΔ486 (lane b) or HypBA1 (lane c) at 37 °C for 16 h. Lane a, control without enzyme; lane 1, l-arabinose standard. B, HPAEC-PAD analysis of the reaction products. Potato lectin was reacted without enzyme (2a), with HypBA2-CΔ486 (2b), or with HypBA1 (2c). Extensin was reacted without enzyme (3a), with HypBA2-CΔ486 (3b) or with HypBA1 (3c).