FIGURE 8.
Comparison of the reactivities of HypBA1 and HypBA2 with glycoproteins. A, TLC analysis of the reaction products. Potato lectin (lane 2), extensin (lane 3), and Ara3-Hyp (lane 4) were incubated with HypBA2-CΔ486 (lane b) or HypBA1 (lane c) at 37 °C for 16 h. Lane a, control without enzyme; lane 1, l-arabinose standard. B, HPAEC-PAD analysis of the reaction products. Potato lectin was reacted without enzyme (2a), with HypBA2-CΔ486 (2b), or with HypBA1 (2c). Extensin was reacted without enzyme (3a), with HypBA2-CΔ486 (3b) or with HypBA1 (3c).