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Transglycosylation activity of HypBA1 in the presence of 1-alkanols. A, HypBA1 was incubated with Ara2-Hyp either in the absence (lane 3) or in the presence of 20% methanol (lane 4), ethanol (lane 5), or 1-propanol (lane 6) at 37 °C for 3 h. Lane 1, l-arabinose; lane 2, Ara2-Hyp. B, purified methyl l-arabinofuranoside was incubated without (lane 1) or with (lane 2) HypBA1 at 37 °C for 16 h. Lane 3, l-arabinose standard. Me, methyl; Et, ethyl; Pr, propyl.
