TABLE 1.

The primers used for site-directed mutagenesis

The positions of the mutated sequences are underlined.

Name	Sequence of oligonucleotide primers
E322A_Forward_Primer	5′-ACCCACGTGGGCGCGTCGTTCACCTACG-3′
E322A_Reverse_Primer	5′-CGTAGGTGAACGACGCGCCCACGTGGGT-3′
E338A_Forward_Primer	5′-CACGATGTACGGTGCGACCTGTGCTTCCG-3′
E338A_Reverse_Primer	5′-GAAGCACAGGTCGCACCGTACATCGTGTCG-3′
E366A_Forward_Primer	5′-CCGACGTGCTGGCGAAGGAACTGTTCAACG-3′
E366A_Reverse_Primer	5′-CGTTGAACAGTTCCTTCGCCAGCACGTCG-3′