FIGURE 6.
Representative ITC data of PaAbf62A and UmAbf62A binding to xylo- and cello-oligosaccharides. A–D, the upper parts of each panel show the raw binding heats, the lower parts show the integrated binding heats minus the dilution control heats fitted to a single-site binding model. Experiments were performed at 25 °C. The concentration of PaAbf62A was 114 μm; and UmAbf62A was 488 and 95 μm. A, PaAbf62A titration with X6 (1.6 mm); B, PaAbf62A titration with C6 (2.2 mm); C, UmAbf62A (488 μm) titration with X6 (5 mm); and D, UmAbf62A (95 μm) titration with C6 (3 mm). In panels A and B, n is the number of binding sites and Ka is the affinity constant both determined by ITC, the values are the mean of three experiments.