TABLE 2.

Kinetic parameters of PaAbf62A and UmAbf62A

Michaelis constant were measured using pNP-Ara as substrate. Inhibition by C6 was measured using pNP-Ara as substrate as described under “Experimental Procedures.”

	PaAbf62A	UmAbf62A
pNP-Ara
Vmaxa	0.61 ± 0.02b	0.06 ± 0.00
kcat (min−1)	22.6 ± 0.0	2.32 ± 0.02
Km (mm)	6.1 ± 0.3b	7.5 ± 0.8
kcat/Km (min−1 mm−1)	3.70 ± 0.06b	0.31 ± 0.03
C6
Ki (mm)	3.40 ± 0.25	NDc

^a V_max is defined as the μmol of product formed per mg of enzyme per min.

^b Result from Couturier et al. (18).

^c ND, no inhibition detected.