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Simvastatin, but not atorvastatin, induces tuberous sclerosis complex 2 (TSC2)-null cell rounding. A total of 1.5 × 105 TSC2-null cells were plated on 6-well plates and treated with 10 μM of atorvastatin or simvastatin added freshly every other day as described in Results. Untreated cell were used as a control. Representative images were taken using a phase-contrast microscope.
