Skip to main content
. Author manuscript; available in PMC: 2014 Sep 17.
Published in final edited form as: Sci Signal. 2013 Sep 17;6(293):ra83. doi: 10.1126/scisignal.2004214

Fig 3. 15(S)-HETE stimulates NADPH oxidase and xanthine oxidase activities and ROS production in mouse peritoneal macrophages and enhances their migration and adhesion.

Fig 3

(A to C) Quiescent mouse peritoneal macrophages were treated with vehicle (Control) or 0.1 μM 15(S)-HETE for the indicated times before being analyzed for (A) NADPH oxidase activity, (B) XO activity, and (C) ROS production. (D) The migration of quiescent macrophages in response to vehicle (Control) or 0.1 μM 15(S)-HETE was measured by the modified Boyden chamber method. (E) Quiescent macrophages were treated with vehicle (Control) or 0.1 μM 15(S)-HETE for 1 hour, labeled with 10 μM BCECF-AM for 30 min, overlaid onto a monolayer of quiescent mouse endothelial cells, and incubated for 2 hours. Adherent cells were determined by measuring fluorescence intensities. Data are means ± SD from three independent experiments. *P < 0.01 versus control.