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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1978 Dec;75(12):6107–6109. doi: 10.1073/pnas.75.12.6107

Adipose conversion of 3T3 cells depends on a serum factor.

W Kuri-Harcuch, H Green
PMCID: PMC393127  PMID: 282628

Abstract

The adipose conversion of 3T3-F442A cells depends on an adipogenic factor in serum. In the presence of this factor, cells grown to confluence become spherical, greatly increase the activity of their lipogenic enzymes, and accumulate triglyceride. In the absence of the adipogenic factor, the cells grow normally, but when they reach confluence they do not become spherical, do not accumulate triglyceride, and do not undergo any increase in activity of lipogenic enzymes. In cattle there is a great deal more of the adipogenic factor in the serum before birth than in the serum of grown animals. The nature of the adipogenic factor suggests that it may play an important role in the development of adipose tissue.

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Selected References

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  1. Eckel R. H., Fujimoto W. Y., Brunzell J. D. Development of lipoprotein lipase in cultured 3T3-L1 cells. Biochem Biophys Res Commun. 1977 Sep 9;78(1):288–293. doi: 10.1016/0006-291x(77)91252-9. [DOI] [PubMed] [Google Scholar]
  2. Green H., Kehinde O. An established preadipose cell line and its differentiation in culture. II. Factors affecting the adipose conversion. Cell. 1975 May;5(1):19–27. doi: 10.1016/0092-8674(75)90087-2. [DOI] [PubMed] [Google Scholar]
  3. Green H., Kehinde O. Spontaneous heritable changes leading to increased adipose conversion in 3T3 cells. Cell. 1976 Jan;7(1):105–113. doi: 10.1016/0092-8674(76)90260-9. [DOI] [PubMed] [Google Scholar]
  4. Grimaldi P., Négrel R., Ailhaud G. Induction of the triglyceride pathway enzymes and of lipolytic enzymes during differentiation in a 'preadipocyte' cell line. Eur J Biochem. 1978 Mar 15;84(2):369–376. doi: 10.1111/j.1432-1033.1978.tb12177.x. [DOI] [PubMed] [Google Scholar]
  5. Kuri-Harcuch W., Green H. Increasing activity of enzymes on pathway of triacylglycerol synthesis during adipose conversion of 3T3 cells. J Biol Chem. 1977 Mar 25;252(6):2158–2160. [PubMed] [Google Scholar]
  6. Kuri-Harcuch W., Wise L. S., Green H. Interruption of the adipose conversion of 3T3 cells by biotin deficiency: differentiation without triglyceride accumulation. Cell. 1978 May;14(1):53–59. doi: 10.1016/0092-8674(78)90300-8. [DOI] [PubMed] [Google Scholar]
  7. Mackall J. C., Lane M. D. Role of pyruvate carboxylase in fatty acid synthesis: alterations during preadipocyte differentiation. Biochem Biophys Res Commun. 1977 Dec 7;79(3):720–725. doi: 10.1016/0006-291x(77)91171-8. [DOI] [PubMed] [Google Scholar]
  8. Mackall J. C., Student A. K., Polakis S. E., Lane M. D. Induction of lipogenesis during differentiation in a "preadipocyte" cell line. J Biol Chem. 1976 Oct 25;251(20):6462–6464. [PubMed] [Google Scholar]
  9. Reed B. C., Kaufmann S. H., Mackall J. C., Student A. K., Lane M. D. Alterations in insulin binding accompanying differentiation of 3T3-L1 preadipocytes. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4876–4880. doi: 10.1073/pnas.74.11.4876. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rothblat G. H., Arbogast L. Y., Ouellette L., Howard B. V. Preparation of delipidized serum protein for use in cell culture systems. In Vitro. 1976 Aug;12(8):554–557. doi: 10.1007/BF02797438. [DOI] [PubMed] [Google Scholar]
  11. Rubin C. S., Lai E., Rosen O. M. Acquisition of increased hormone sensitivity during in vitro adipocyte development. J Biol Chem. 1977 May 25;252(10):3554–3557. [PubMed] [Google Scholar]
  12. Russell T. R., Ho R. Conversion of 3T3 fibroblasts into adipose cells: triggering of differentiation by prostaglandin F2alpha and 1-methyl-3-isobutyl xanthine. Proc Natl Acad Sci U S A. 1976 Dec;73(12):4516–4520. doi: 10.1073/pnas.73.12.4516. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Wise L. S., Green H. Studies of lipoprotein lipase during the adipose conversion of 3T3 cells. Cell. 1978 Feb;13(2):233–242. doi: 10.1016/0092-8674(78)90192-7. [DOI] [PubMed] [Google Scholar]

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