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. 2014 Feb 18;12:16. doi: 10.1186/1477-7827-12-16

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Copyright © 2014 Russo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Confocal images of mt distribution, ROS levels and mt/ROS colocalization of dromedary camel oocytes. Mitochondrial distribution patterns and intracellular reactive oxygen species (ROS) localization in dromedary camel oocytes at GV (Panel A), MI (Panel B), and MII (Panel C) stage observed after staining with MitoTracker Orange CMTM Ros, H₂DCF-DA and Hoechst 33258. An immature GV stage oocyte showing homogeneous mt distribution pattern of small mitochondrial aggregates (Panel A), a MI oocyte showing heterogeneous distribution of mitochondria (pericortical tubular network, PCTN; Panel B), and a MII oocyte showing heterogeneous distribution of mitochondria (perinuclear/pericortical tubular network, PN/PCTN; Panel C) are shown. For each sample, corresponding bright field (A1, B1, C1), UV light (A2, B2, C2) and confocal images showing mitochondrial distribution pattern (A3, B3, C3) intracellular ROS localization (A4, B4, C4), mitochondrial/ROS merge (A5, B5, C5) and the mitochondrial/ROS colocalization scatter plot (A6, B6, C6) are shown. The scale bar represents 60 μm.