Table 1.
Reference | Definition of EGFR overexpression | Tumors overexpressing EGFR (%) | Reference | Definition of HER2 overexpression | Tumors overexpressing HER2 (%) |
---|---|---|---|---|---|
Onn et al. [145] | 2 or 3 staining by IHC | 60 | Slamon et al. [151] | Twofold or more HER2 gene by Southern blotting | 30 |
Selvaggi et al. [146] | 2+ or 3+ staining by IHC | 37 | Paik et al. [152] | Definite membrane staining by IHC in tumor cell | 29 |
Ohtsuka et al. [147] | 2+ or 3+ by Western blotting | 40 | Owens et al. [153] | Twofold or more of HER2 gene by FISH | 23 |
Dancer et al. [148] | Twofold or more EGFR gene by FISH | 65 | Owens et al. [153] | 2+ or higher result by IHC | 20 |
Bloomston et al. [149] | 1+ or higher staining by IHC | 69 | Seshadri et al. [154] | Twofold or more HER2 gene by slot blotting | 21 |
Thybusch-Bernhardt et al. [150] | Positive staining by IHC | 33 | Andrulis et al. [155] | Twofold or more HER2 gene by RT-PCR | 20 |
FISH fluorescent in situ hybridization, IHC immunohistochemistry, RT-PCR real-time polymerase chain reaction