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. 1978 Dec;75(12):6125–6129. doi: 10.1073/pnas.75.12.6125

Detection of viral sequences of low reiteration frequency by in situ hybridization.

M Brahic, A T Haase
PMCID: PMC393131  PMID: 216014

Abstract

The sensitivity of in situ hybridization has been increased at least 10-fold by hybridizing in cDNA excess, by increasing the diffusion of the cDNA through the cells, by hybridizing at optimum temperature, and by stabilizing hybrids during autoradiography. Saturation of intracellular RNA with [3H]cDNA has been achieved. The assay is quantitative. In situ hybridization has been used to detect and quantitate visna virus RNA in infected cells. By using [3H]cDNA with specific activity of 2 X 10(8) dpm/micrograms and conditions that reduce background to negligible levels, 10--20 copies of viral RNA per cell can be detected and quantitated after 2 days of autoradiographic exposure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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