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. 2014 Jan 27;13:15. doi: 10.1186/1476-4598-13-15

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Copyright © 2014 Ding et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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CD133 acts as a regulator of the mesenchymal phenotype. (A) A heatmap of the DNA microarray demonstrates the distinct molecular profiles of Capan1M9 and shCD133M9 cells. (B) A heatmap of EMT-related genes with a Z-score greater than 2 distinguished between Capan1M9 and shCD133M9 cells. (C) Validation of the levels of the EMT-related proteins fibronectin, N-cadherin, and E-cadherin in Capan1M9 and shCD133M9 cells by Western blot. (D) Validation of EMT-related genes in Capan1M9 with shCD133M9 cells by real-time RT-PCR.