Figure 6.

Quantitative real-time PCR confirmation of differential expression. In small vs. large fish (i.e. small: large expression ratios shown) in the Sept. lot for differentially expressed genes identified by microarray analysis (left panel) and by qPCR (right panel) in the A) liver: complement C1q-like protein 4 precursor (C1QL4), insulin-like growth factor 1 binding protein (IGF1BP), and hemoglobin subunit alpha (HBA1) and B) muscle: glyceraldehyde-3-phosphate dehydrogenase (GAPDH), adp/atp translocase 2 (ANT2), max protein (MAX). (*Significantly different when the large fish were compared to the small fish by a t-test, P ≤ 0.05).