Figure 1.

Two-dimensional-DIGE results. (A) Hierarchical clustering of RPL10[H213Q] mutation carriers (MUT) and wild-type allele carriers (WT) including spots with P-value ≤0.03 only. These 33 spots separated MUT from WT and, in addition, separately clustered families. Two distinct protein clusters (A, B) are visible with cluster B being more affected in family 440. Asterisks mark samples from ASD patients. (B) Best hierarchical clustering between ASD and controls (CTRL) was observed when selecting spots with P-value ≤0.05 (N = 52). Two distinct clusters are down- (A) and up-regulated (B), respectively, in the ASD cohort. For better visualization missing values in A and B omitted during calculations are shown in black. (C) In yeast, proteins differentially regulated under hydrogen peroxide (H₂O₂) induced oxidative stress overlap with a set of proteins (marked in grey) differentially regulated in the rpl10 deficient yeast strain L10/ΔL10 (all P-values ≤0.05, comparison for both conditions are to wild-type yeast). (D) Co-expression networks of identified candidates based on biological functions related to GO-terms. RPL10 was manually spiked into each network and is co-regulated in every module. Gene-expression data are retrieved from the GeneMania database. For details on methods and network construction see the Materials and Methods section. Symbols used in protein names in B and C: ‘&’ = a mixture of both proteins was identified; ‘|’ = MS data were not able to clearly distinguish between named protein isoforms. ASD, autism spectrum disorders; DIGE, difference gel electrophoresis; GO, Gene Ontology database; MS, mass spectroscopy.