Figure 2.

In vitro culture and immunophenotyping of islet MSC-like cells. A: Islet stromal cells isolated from NOD mice (pooled from four to five mice) and cultured in vitro for different periods. B: After two to three passages, cells were trypsinized and analyzed by flow cytometry using CD105, Sca-1, CD31, CD44, CD90, CD45, and CD81 antibodies. Broken line is for isotype control, and shaded area represents positive marker expression. Data represent three (A) or five to six (B) independent experiments. Max, maximum.