Figure 4.

Correlation of EXO-induced IFN-γ secretion with serum EXO levels in prediabetic NOD mice. A: Splenocytes were isolated from NOD mice (three to four mice/group) at 4 and 8 weeks of age and stimulated for 72 h in vitro with iMSC-EXOs (5 µg/mL). Culture supernatants were tested for released IFN-γ using CBA assay. B: Serum EXOs were isolated by the ExoQuick kit. EXO concentration was determined by measuring CD81 protein by ELISA. Data show OD values for individual mice at different ages, with the horizontal line indicating mean values. Experiments were repeated four times (A) or three times (B) with similar results.