Skip to main content
. 2014 Feb 13;63(3):1103–1114. doi: 10.2337/db13-0927

Figure 5.

Figure 5

Blocking IL-1β signaling blunts conditioned medium–induced inflammasome activity in vitro. A–F: Bone marrow–derived Mp from wild-type (WT) mice either left nonstimulated (Non) or stimulated with day 10 db/db wound-conditioned medium (CM) along with control IgG or IL-1β blocking antibody (IL1ab), and expression of IL-1β, IL-18, PI-9, and caspase-12 measured by real-time PCR. G and H: Bone marrow–derived Mp from wild-type mice either left nonstimulated or treated with day 10 db/db wound CM along with control IgG or IL-1β blocking antibody and IL-1β and IL-18 release measured using ELISA. I–N: Bone marrow–derived Mp from WT and IL-1R1 knockout (ko) mice stimulated with day 10 db/db wound CM and expression of IL-1β, IL-18, PI-9, and caspase-12 measured by real-time PCR. O and P: Bone marrow–derived Mp from WT and IL-1R1ko mice stimulated with day 10 db/db CM and IL-1β and IL-18 release measured using ELISA. For all graphs, bars = mean ± SD, n = 5–6 in two independent experiments. Data compared between groups using ANOVA on ranks. *Mean value significantly different from that for nonstimulated controls; **mean value significantly different from that for wound-conditioned medium + IgG-treated samples; #mean value significantly different from that for nonstimulated controls; ##mean value significantly different from that for wound-conditioned medium–treated wild-type Mp at same time point; P < 0.05. Diff, difference.