Table 1. Strains and Plasmids Used in This Worka.
| strain or plasmid | relevant characteristics | ref |
|---|---|---|
| strains | ||
| L. reuteri | ||
| DSMZ 17509 | source for l-AI and d-XI encoding genes | DSMZ |
| L. plantarum | ||
| WCFS1 | wild type | (49) |
| TLG02 | Δalr, d-alanine auxotroph | (19) |
| E. coli | ||
| MB2159 | d-alanine auxotroph, cloning host | (50) |
| NEB5α | cloning host | New England Biolabs |
| BL21 Star (DE3) | expression host | Invitrogen |
| plasmids | ||
| pET-21a(+) | Ampr, T7 promoter, C-terminal His-Tag | Novagen |
| pSIP409 | Ermr, pSIP401 derivative, PsppQ | (25) |
| pSIP609 | pSIP409 derivative, alr replaced with erm | (19) |
| pPW002 | l-AI from L. reuteri in pET-21a(+) | this work |
| pPW004 | d-XI from L. reuteri in pET-21a(+) | this work |
| pPS1 | pSIP609 derivative, NcoI replaced with NheI | this work |
| pPW-015 | pSIP409 derivative, NcoI replaced with NheI | this work |
| pPS2 | l-AI and d-XI from L. reuteri in pPS1 | this work |
| pPS3 | l-AI and d-XI from L. reuteri in pPW-015 | this work |
| pPW116 | l-AI from L. reuteri in pJET1.2 | this work |
| pPW114 | d-XI from L. reuteri in pJET1.2 | this work |
a
Ampr, ampicillin resistance; Ermr, erythromycin resistance; alr, alanine racemase encoding gene; erm, erythromycin resistance gene.