Table 2. Primers Used in This Studya.
| primer | sequence (5′–3′) |
|---|---|
| araA_LbReu_fwd1 | ATGGATATTAAGAATTACGAATTTTGGTTT |
| araA_LbReu_rev1 | CTATTCAATAAAATCAACTTTAACA |
| xylA_LbReu_fwd1 | TCATCATGATAAGGATAAAGTTTTGG |
| xylA_LbReu_rev1 | ATTGCAATCGTTGTTCCATAAAGCCAATC |
| pSIP_SD_NheI_fwd1 | TACGTCCTGTAGAAACCCCAAC |
| pSIP409_SD_NheI_rev1 | CTACAGGACGTAGCTAGCCTAAAATCTCCTTGT |
| AraReuNheI_fwd1 | TTTTCGCTAGCATGGATATTAAGAATTACG |
| AraReuEcoRI_His6 | AACGCGAATTCGTTTCAATAAAATCAACTT |
| XylReuNheI_fwd1 | ATTTTGCTAGCATGGCTGATTTATGGA |
| XylReuEcoRI_His6 | CCGTGGAATTCGTCTTAGCAAGCGTTTC |
| AraReu_OE_rev1 | TAAAACTCCTGAACTAGTGGTGGTGGTGGTGGTGTTCAATAAAATCAAC |
| XylReu_OE_fwd1 | TTCAGGAGATTTTAATGGCTGATTTATGGAATAA |
| XylReu_OE_rev1 | CGAATGAATTCTTAGTGGTGGTGGTGGTGGTGCTTAGCAAGCGTTTC |
a
The restriction sites are underlined.