FIG. 1.

Stopping redox modulation leads to finite autoreactive immune cell control. BDC-2.5.TCR.Tg mice were treated for 7 days i.p. with MnP or HBSS at 10 mg/kg. (A) On day 8, splenocytes were harvested and adoptively transferred intravenously into NOD.scid recipients. Recipients were monitored by glucosuria and blood glucose and considered diabetic after two consecutive blood glucose readings of >300 mg/dL. n=3 transfers/group, p<0.0001. (B) On day 8, splenocytes were harvested for in vitro stimulation with 2.5 mimotope±MnP. At 48–96 h, supernatants were collected and used in an IFN-γ ELISA. Data show the average of independent experiments performed in triplicate from n=5 mice/group, *p<0.05. BDC, Barbara Davis Center; ELISA, enzyme-linked immunosorbent assay; HBSS, Hank's balanced salt solution; i.p., intraperitoneal; NOD, nonobese diabetic; Tg, transgenic; IFN, interferon.