Antiproliferative effects of 17-AAG, novobiocin, 174, and 401 in a panel of cancer cell lines. (A–D) Antiproliferative effects of 17-AAG, novobiocin, 174, and 401 in A549 (■), PC3-MM2 (●), and HCT116 (▲) cells. These data are the mean±SEM from three independent experiments performed in duplicate (n=3) and are represented as percent growth, where 100 to 0 is the antiproliferative response and 0 to -100 is the cytotoxic response. (E) Western blots for client protein degradation of survivin, AKT, and cdc25C as well as the induction of heat-shock response in A549 cells after treatment with 401 along with positive (17-AAG and novobiocin) and negative (vehicle, 0.25% DMSO) controls. These results are representative of three independent experiments (n=3).