Table 1.
Reverse-Transcription Polymerase Chain Reaction Primers and Probes
| Human | Swine | |
|---|---|---|
| BMP2 | ||
| Forward | CCA GAC CAC CGG TTG GAG A | GGC TGG AGA GGG CAG CCA |
| Reverse | TTC CAA AGA TTC TTC ATG GTG G | CTC ATT TCT GGC AGT TCT TCC |
| Probe | F-AGC CAG CCG AGC CAA CAC TGT GC-Q | F-TGG CCA ACA CCG TGC GCA GCT TCC A-Q |
| Col1A1 | ||
| Forward | AAC AGC CGC TTC ACC TAC AG | GCC AAG AAG AAG ACA TCC CA |
| Reverse | TCA ATC ACT GTC TTG CCC CA | TTT CCA CAC GTC TCG GTC AT |
| Probe | F-TCG ATG GCT GCA CGA GTC ACA CCG-Q | F-AGT CAC CTG CGT ACA GAA CGG CCT C-Q |
| Col2A1 | ||
| Forward | CAA TAG CAG GTT CAC GTA CAC | TGT CAC GGC CAG GAT GTC CA |
| Reverse | TCG ATA ACA GTC TTG CCC CA | GGC TTC CAC ACA TCC TTA TCA |
| Probe | F-AGG ATG GCT GCA CGA AAC ATA CCG-Q | F-ACC TCT GCC CAT CCT GCA CGC AGC-Q |
| 18S | ||
| Forward | AGA AAC GGC TAC CAC ATC CA | AGA AAC GGC TAC CAC ATC CA |
| Reverse | CTC GAA AGA GTC CTG TAT TGT | CTC GAA AGA GTC CTG TAT TGT |
| Probe | F-AGG CAG CAG GCG CGC AAA TTA C-Q | F-AGG CAG CAG GCG CGC AAA TTA C-Q |
F, 5′-fluorescein (FAM) and 5′-tetrachloro-fluorescein (TET) in 18S; Q, quencher (TAMRA).