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. Author manuscript; available in PMC: 2015 Jan 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 Dec 1;274(2):283–292. doi: 10.1016/j.taap.2013.11.015

Figure 1.

Figure 1

Fluorescence microscopy visualization of BDE-47-stimulated dichlorofluorescein (DCF) fluorescence, an index of reactive oxygen species production, in a first trimester human extravillous trophoblast cell line, HTR-8/SVneo. HTR-8/SVneo cells were pretreated for 1 h with or without 1 mM deferoxamine mesylate (DFO), and then received no further treatment (NT, non-treated control) or were exposed to DMSO (solvent control), 20 μM BDE-47 or 100 μM tert-butyl hydroperoxide (TBHP; positive control) for 4 h. Subsequently, the cells were loaded with the non-fluorescent pro-dye carboxy-H2DCF-DA for 1 h, counterstained with CellMask™ Deep Red plasma membrane dye, and photographed using an epifluorescence microscope. The top panel shows representative images of intracellular DCF fluorescence, and the bottom panel show corresponding Deep Red membrane staining. Representative images of 3 independent experiments.