Skip to main content
. 2013 Jul 29;2:142. Originally published 2013 Jun 19. [Version 2] doi: 10.12688/f1000research.2-142.v2

Figure 11. Molecular analyses of gun4-II complements.

Figure 11.

( A) A schematic diagram of the GUN4-pDBle construct. Primers used for PCR are shown on the map. ( B) Genomic DNA PCR analyses with GUN4 cloning primers (product size: 969 bp). Lane 1: gun4-II; Lane 2: 4A+; Lane 3: gun4-19; Lane 4: gun4-27. ( C) RT-PCR analyses with GUN4 cloning primers (product size: 802 bp). Lane 1: gun4; Lane 2: 4A+; Lane 3: gun4-19; Lane 4: gun4-27. ( D) Genomic PCR analyses using a PsaD 5′ UTR specific forward primer with a GUN4 cloning reverse primer (product size: 976 bp). Lane 1: gun4-II; Lane 2: 4A+; Lane 3: gun4-19; Lane 4: gun4-27. Primer sequences are shown in Table 5.