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. 2013 Jul 29;2:142. Originally published 2013 Jun 19. [Version 2] doi: 10.12688/f1000research.2-142.v2

Table 4. List of primers used for transcript analysis of GUN4 and GUN4 neighboring genes in 6F14.

These primers were used to generate the data in Figure 7. The gene loci numbers in Phytozome for the three neighboring genes of GUN4 on chromosome 5 and the control actin gene on chromosome 13 are: HYP1 [Cre05.g246750], HYP2 [g5195] and SOXE [Cre05.g246900] and Actin (Cre13.g603700), respectively.

Primer name Sequence of primer Purpose
F2 5´-ACGACACCACCTTCAACTCCATCA-3´ Actin
R2 5´-TTAGAAGCACTTCCGGTGCACGAT-3´ Actin
NupF4 5´-TGTATGAACTCTGAGCAGGCGACA-3´ HYP1
Nup98R2 5´-CCTGCCGTATGTCGTGCACAAAC-3´ HYP1
3F 5´-TGTGGAGAAGAAGAAGTCCGGCAA-3´ GUN4
8R 5´-GGTGCAATCTTTGCAAGATGCGGT-3´ GUN4
HypF2 5´-TTCCTGGCTACTGCCGTATTCGCA-3´ HYP2
H6F 5´-GCCTTGCCGTGCTGCATTAGAATA-3´ HYP2
PB120 5´-GCACGGATGGCAAGTACATG-3´ SOXE
PB121 5´-CTACTTCACTGCCCTGGAGTTT-3´ SOXE