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. 2013 Jul 29;2:142. Originally published 2013 Jun 19. [Version 2] doi: 10.12688/f1000research.2-142.v2

Table 5. List of primers used for cloning and complement testing.

These primers were used in the experiments that generated the data in Figure 8 and Figure 11 and were also used for GUN4 gene amplification for cloning.

Primer name Sequence of primer Purpose
GUN4F1 5´-GGAATTCCATATGCTGGCCCAAACACACACT-3´ Amplification of GUN4 for cloning
GUN4R1 5´-CCGGAATTCTTAGAACAGCGACTGTGTCCGCC-3´ Amplification of GUN4 for cloning
and for complement testing
PsaDF1 5´-CCACTGCTACTCACAACAAGCCCA-3´ Complement testing