Table 5. List of primers used for cloning and complement testing.
Primer name | Sequence of primer | Purpose |
---|---|---|
GUN4F1 | 5´-GGAATTCCATATGCTGGCCCAAACACACACT-3´ | Amplification of GUN4 for cloning |
GUN4R1 | 5´-CCGGAATTCTTAGAACAGCGACTGTGTCCGCC-3´ | Amplification of
GUN4 for cloning
and for complement testing |
PsaDF1 | 5´-CCACTGCTACTCACAACAAGCCCA-3´ | Complement testing |