Table 2.
Primers and TaqMan probes for KIAA1549-BRAF fusion gene variants
| Gene fusion | Primer | TaqMan probe |
|---|---|---|
|
KIAA1549-BRAF (KIAA exon 13 - BRAF exon 11) forward |
GGGTCCCCAGTAAGATCCAG |
ATCGCCATGCAGCCGATCCCGGCACCT |
|
KIAA1549-BRAF (KIAA exon 13 - BRAF exon 11) reverse |
CTCGAGTCCCGTCTACCAAG |
|
|
KIAA1549-BRAF (KIAA exon 15 - BRAF exon 9) forward |
CGTCCACAACTCAGCCTACATC |
ACCACAGGTTTGTCTGC |
|
KIAA1549-BRAF (KIAA exon 15 - BRAF exon 9) reverse |
CCTGGAGATTTCTGTAAGGCTTTC |
|
|
KIAA1549-BRAF (KIAA exon 15 - BRAF exon 11) forward |
AGCGATGGCACCTACAGGA |
CGTCCACAACTCAGCCTACATCGGATGCCCA |
|
KIAA1549-BRAF (KIAA exon 15 - BRAF exon 11) reverse |
TCATCACTCGAGTCCCGTCT |
|
|
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 9) forward |
CCAGACGGCCAACAATCC |
ACCACAGGTTTGTCTGC |
|
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 9) reverse |
CCTGGAGATTTCTGTAAGGCTTTC |
|
|
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 10) forward |
CAGTGGGGGTCCTTCTACAG |
AGCCCAGACGGCCAACAATCCCTGCAG |
|
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 10) reverse |
CTTCCTTTCTCGCTGAGGTC |
|
|
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 11) forward |
AGTGGGGGTCCTTCTACAGC |
AGCCCAGACGGCCAACAATCCCTGCAG |
|
KIAA1549-BRAF (KIAA exon 16 - BRAF exon 11) reverse |
CATGCCACTTTCCCTTGTAG |
|
|
KIAA1549-BRAF (KIAA exon 17 - BRAF exon 10) forward |
GAATGACTCCCCCGACG |
ACCACAGGTTTGTCTGCTACCCCCCCTGC |
|
KIAA1549-BRAF (KIAA exon 17 - BRAF exon 10) reverse |
AGGCTTTCACGTTAGTTAGTGAGC |
|
|
KIAA1549-BRAF (KIAA exon 18 - BRAF exon 10) forward |
TGCTGCCAGAGGGATCTACTC |
ACCACAGGTTTGTCTGC |
|
KIAA1549-BRAF (KIAA exon 18 - BRAF exon 10) reverse |
CCTGGAGATTTCTGTAAGGCTTTC |
|
|
KIAA1549-BRAF (KIAA exon 19 - BRAF exon 9) forward |
CCAGGCTGGCCTTCGTAC |
ACCACAGGTTTGTCTGC |
| KIAA1549-BRAF (KIAA exon 19 - BRAF exon 9) reverse | CCTGGAGATTTCTGTAAGGCTTTC |