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. 2014 Feb 19;34(8):2768–2773. doi: 10.1523/JNEUROSCI.4402-13.2014

Figure 3.

Figure 3.

Effects of a single oral dose (0.75 mg/kg) of MPH on PNE induced spontaneous locomotor activity in male and female F2 mice derived from female founders. Locomotor activity was assayed from 17:00–09:00 h (A–D). MPH or saline (SAL) was gavaged at 19:00 h. Cumulative activity in the PNE (E) and SAC (F) groups was analyzed for the 12 h lights-off period from 19:00–07:00 h. Data are shown as means ± SEM. Hourly measurements showed that both male (A) and female (B) mice derived from PNE founders showed lower activity upon MPH administration compared with SAL administration. In the SAC group, MPH administration did not produce significant effects on the hourly activity compared with SAL administration in male (C) or female (D) mice. Cumulative activity over the entire 12 h lights-off period showed significant decreases in the PNE male and female mice (E) receiving MPH compared with SAL, whereas MPH had no significant effect on male or female mice in the SAC group (F). Cumulative activity showed significant effects of drug treatment in the PNE group (ANOVA: F(3,28) = 6.3; p = 0.003). Tukey–Kramer multiple-comparison test revealed significant differences in cumulative activity between SAL and MPH treatments for male and female mice (p < 0.05). ANOVA did not reveal significant changes in cumulative activity in the SAC group (F(3,37) = 1.02, p = 0.39).