Skip to main content
. 2014 Feb 19;34(8):3079–3089. doi: 10.1523/JNEUROSCI.2286-13.2014

Figure 5.

Figure 5.

PINK1 mediates protein translation. A, Loss of PINK1 leads to decreased HIF-1α translation. PINK1+/+ and PINK1−/− MEFs were starved for 30 min under either 21% or 0.1% O2 followed by a 10 min pulse with 35S-met/cys-containing media under either 21% or 0.1% O2. Radiolabeled HIF-1α was analyzed by radiography and the density of the HIF-1α was measured with Quantity One 1-D Analysis Software (Bio-Rad). A representative figure is shown from five independent runs. B–D, PINK1 increases the relative ratio of IRES-dependent to cap-dependent translation. PINK1+/+ and PINK1−/− MEFs were transfected with pcDNA3-RLUC-POLIRES-FLUC and then treated with 0.1% O2 for 24 h. Cell lysates were analyzed for (B) IRES activity by firefly luciferase, (C) cap activity by Renilla luciferase, and (D) the ratio of IRES/CAP. Samples for each assay were normalized to protein concentration and readings from three independent experiments were pooled. *p value < 0.05, **p value < 0.01, #p value <0.001.