Skip to main content
. 2014 Feb 19;34(8):3079–3089. doi: 10.1523/JNEUROSCI.2286-13.2014

Figure 7.

Figure 7.

4E-BP1 overexpression rescues translation impairments in PINK1−/− MEFs. A–C, PINK1+/+ and PINK1−/− MEFs were cotransfected with pcDNA3-RLUC-POLIRES-FLUC and vector or FLAG-4E-BP1 plasmid and then treated with 0.1% O2 for 24 h. Cell lysates were analyzed for (A) IRES activity by firefly luciferase, (B) cap activity by Renilla luciferase, and (C) the ratio of IRES/CAP. Samples for each assay were normalized to protein concentration and readings from three independent experiments were pooled. *p value < 0.05, **p value < 0.01, #p value <0.001.